CHIKV RNA in the dLN localizes to MARCO+ LECs. WT C57BL/6 mice were mock-inoculated (n = 3) or inoculated with 103 PFU of CHIKV (n = 3) in the left rear footpad. At 24 hpi, the draining popliteal LN was collected and enzymatically digested into a single cell suspension. Cells were enriched for CD45- cells and analyzed by scRNA-seq as described in the materials and methods. P-values were calculated using a two-sided Wilcoxon rank-sum test with Bonferroni correction.